Method of increasing plant transformation frequency using modified strains of Agrobacteria

US9617551B2 · kind B2 · utility

Assignee

• DOW AGROSCIENCES LLC · US

Inventors

• Donald J. Merlo · Carmel, US
• Sean M. Russell · Carmel, US
• Diane M. Retallack · Poway, US
• Aaron T. Woosley · Fishers, US
• Thomas Meade · Saunderstown, US
• Kenneth E. Narva · San Diego, US

Key dates

• Filing date: Jul 29, 2011
• Grant date: Apr 11, 2017
• Expiry date: Oct 30, 2033

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A40/146
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.