Selective nucleic acid fragment recovery
US9624252B2 · kind B2 · utility
0Cited by
3References
11Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 1, 2014 |
| Grant date | Apr 18, 2017 |
| Priority date | — |
| Expiry date | Feb 13, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention provides methods and kits for nucleic acid purification and fragment selection and recovery. By adjusting salt concentrations and/or pH of the binding buffers, only nucleic acid fragments with desired size ranges are able to reversibly and non-specifically bind to a solid surface in certain salt concentrations and/or pH conditions, and can be subsequently eluted and/or recovered from the solid surface in water and/or a low salt eluting buffer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.