In vivo proteomic mapping
US9624524B2 · kind B2 · utility
1Cited by
3References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 15, 2012 |
| Grant date | Apr 18, 2017 |
| Priority date | — |
| Expiry date | Jun 15, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2570/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The application discloses methods, materials, and compositions for the labeling of molecules, for example, proteins, in living cells or in subcellular compartments of living cells. In particular, the application relates to proteomic analysis methods; materials and compositions and means based on direct tagging of unknown proteins with tagging enzymes (such as biotin ligase or a peroxidase) within the vicinity of a tagging substrate (such as a tyramide) within living cells, with optional targeting to specific subcellular locations by expression of genetic constructs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.