Nanogrid rolling circle DNA sequencing
US9624538B2 · kind B2 · utility
112Cited by
14References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 14, 2008 |
| Grant date | Apr 18, 2017 |
| Priority date | — |
| Expiry date | Nov 1, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6874
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.