Aflatoxin nanobody immunoabsorbent and immunoaffinity column and preparation method and use thereof

US9631010B2 · kind B2 · utility

Assignee

• OILCROPS RESEARCH INSTITUTE OF CHINESE ACADAMY OF AGRICULTURE SCIENCES · CN

Inventors

• Peiwu Li · Hubei, CN
• Qi Zhang · Beijing, CN
• Yanru Wang · Beijing, CN
• Zhaowei Zhang · Hubei, CN
• Xiaoxia Ding · Hubei, CN

Key dates

• Filing date: Jul 20, 2016
• Grant date: Apr 25, 2017
• Expiry date: Jul 20, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/38
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An aflatoxin nanobody immunoabsorbent and immunoaffinity column and preparation method and use thereof. The immunoabsorbent comprises a solid phase carrier and aflatoxin B1 nanobody 2014AFB-G15 coupled with the solid phase carrier. The 50% inhibiting concentration IC50 of aflatoxin B1 nanobody 2014AFB-G15 to aflatoxin B1 is 0.66 ng/mL, and the cross-reactivity of aflatoxin B1 nanobody 2014AFB-G15 to aflatoxins B2, G1, G2, and M1 are respectively 22.6%, 10.95%, 32.1% and 26%. The amino acid sequence of aflatoxin B1 nanobody 2014AFB-G15 is as depicted by SEQ ID NO: 7, and the coding gene sequence thereof is as depicted by SEQ ID NO: 8. The aflatoxin nanobody immunoaffinity column can be used for purification and concentration of sample extract prior to computer testing, and the immunoaffinity column can be reused repeatedly.