Chemically modified ligase cofactors, donors and acceptors

US9631227B2 · kind B2 · utility

Assignee

• TriLink BioTechnologies, LLC · US

Inventors

• Natasha Paul · Encinitas, US
• Sabrina Shore · San Diego, US
• Jonathan Shum · Richmond, US
• Alexandre Lebedev · San Diego, US
• Gerald Zon · Redwood City, US

Key dates

• Filing date: Mar 15, 2013
• Grant date: Apr 25, 2017
• Expiry date: Jul 20, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/66
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process.