Methods of measuring fluorescence lifetime using a flow cytometer
US9632030B1 · kind B1 · utility
4Cited by
15References
19Claims
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Key dates
| Filing date | Nov 5, 2013 |
| Grant date | Apr 25, 2017 |
| Priority date | — |
| Expiry date | Oct 19, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N15/1433
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining fluorescent lifetime of a fluorescent particle in a flow cytometer comprising calculating a point on a digitized scatter waveform and a corresponding point on a digitized fluorescence waveform using the same method and calculating the time delay if any between the calculated point on the digitized scatter waveform and the calculated point on the fluorescent waveform to determine the fluorescent lifetime of the fluorescent particle with digitized data collected from a flow cytometer having an unmodulated light source.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.