Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound

US9637766B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• James Kirby · Berkeley, US
• Jeffrey L. Fortman · San Francisco, US
• Minobu Nishimoto · Corona, US
• Jay D. Keasling · Berkeley, US

Key dates

• Filing date: Jun 17, 2016
• Grant date: May 2, 2017
• Expiry date: Jun 17, 2036

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.