Patent · US Active

Glycosylated modified flavin adenine dinucleotide-dependent glucose dehydrogenases, compositions thereof as well as methods of making and using the same

US9637774B2 · kind B2 · utility

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0References

18Claims

0Family size

Assignee

Roche Diabetes Care, Inc. · US

Inventors

Hartmut Duefel · Schlehdorf, DE
Thomas Meier · Cherbourg, FR
Michael Tacke · Munich, DE

Key dates

Filing date	Oct 30, 2014
Grant date	May 2, 2017
Priority date	—
Expiry date	Mar 10, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y101/05
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Compositions, devices, kits and methods are disclosed for assaying glucose with a glycosylated, modified flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), variant thereof or an active fragment thereof, where at least one asparagine residue at positions N2, N168 and N346 of mature, wild-type A. oryzae FAD-GDH according to SEQ ID NO:2 is substituted by one or more amino acids not suitable for glycosylation, thereby eliminating or inactivating, respectively, a potential glycosylation site at this position.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.