Indirect antigen-specific T cell recognition assay

US9638689B2 · kind B2 · utility

Assignee

• Miltenyi Biotec, Inc. · DE

Inventors

• Lan Tong · Huizhou, CN
• Peter Jahn · Leverkusen, DE
• Mario Assenmacher · Bensberg, DE

Key dates

• Filing date: Mar 7, 2014
• Grant date: May 2, 2017
• Expiry date: Dec 3, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/70596
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides a method for analyzing simultaneously multiple human antigen-specific cell populations of a sample, the sample comprising B cells and antigen-specific cells, the method comprising a) separation of B cells from said sample, b) dividing the B cells into n sub-samples, c) differentially labeling the B cells of said sub-samples, wherein at least n-1sub-samples are labeled, d) pulsing of the B cells of each sub-sample with single or multiple peptides, e) pooling of the labeled and peptide-pulsed B cells with cells of said sample comprising said antigen-specific cells, f) co-cultivation of the cells of step e), g) flow cytometry analysis of the B cells with regard to their cell number and CD83 expression, thereby determining the potency of said antigen-specific cells in said sample.