Microfluidic device with reaction sites configured for blind filling

US9643178B2 · kind B2 · utility

Assignee

• FLUIDIGM CORPORATION · US

Inventors

• Marc A. Unger · San Mateo, US
• Ian D. Manger · San Francisco, US
• Michael Lucero · South San Francisco, US
• Yong Yi · San Francisco, US
• Emily Miyashita-Lin · San Francisco, US
• Anja Wienecke · Burlingame, US
• Geoffrey Facer · San Francisco, US

Key dates

• Filing date: Apr 4, 2012
• Grant date: May 9, 2017
• Expiry date: Aug 1, 2033

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.