Patent · US Active

Production of proteins and polypeptides

US9644012B2 · kind B2 · utility

3Cited by

2References

18Claims

0Family size

Assignee

SPIBER TECHNOLOGIES AB · SE

Inventors

Jan Johansson · Stockholm, SE
Anna Rising · Uppsala, SE
My Hedhammar · Stockholm, SE
Kerstin Nordling · Knivsta, SE

Key dates

Filing date	Oct 27, 2010
Grant date	May 9, 2017
Priority date	—
Expiry date	Nov 26, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/62
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method of producing a desired non-spidroin protein or polypeptide is comprising the steps of expressing in a suitable host a fusion protein, obtaining a mixture containing the fusion protein, and optionally isolating the fusion protein. The fusion protein is comprising at least one solubility-enhancing moiety which is derived from the N-terminal (NT) fragment of a spider silk protein. It is further comprising at least one moiety which is a desired non-spidroin protein or polypeptide. Each solubility-enhancing moiety is linked directly or indirectly to the desired protein or polypeptide moiety.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.