Apolipoprotein nanodiscs with telodendrimer

US9644038B2 · kind B2 · utility

Assignees

• The Regents of the University of California · US
• Lawrence Livermore National Security, LLC · US

Inventors

• Juntao Luo · Jamesville, US
• Wei He · Audubon, US
• Kit S. Lam · Davis, US
• Paul Henderson · Dublin, US
• Matthew A. Coleman · Dallas, US
• R. Holland Cheng · Davis, US
• Li Xing · Davis, US

Key dates

• Filing date: Dec 19, 2012
• Grant date: May 9, 2017
• Expiry date: Aug 21, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/775
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides a nanodisc with a membrane scaffold protein. The nanodisc includes a membrane scaffold protein, a telodendrimer and a lipid. The membrane scaffold protein can be apolipoprotein. The telodendrimer has the general formula PEG-L-D-(R)n, wherein D is a dendritic polymer; L is a bond or a linker linked to the focal point group of the dendritic polymer; each PEG is a poly(ethylene glycol) polymer; each R is and end group of the dendritic polymer, or and end group with a covalently bound hydrophobic group, hydrophilic group, amphiphilic compound, or drug; and subscript n is an integer from 2 to 20. Cell free methods of making the nanodiscs are also provided.