RNase H-based assays utilizing modified RNA monomers
US9644198B2 · kind B2 · utility
11Cited by
47References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 5, 2013 |
| Grant date | May 9, 2017 |
| Priority date | — |
| Expiry date | Aug 5, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention pertains to novel oligonucleotide compounds for use in various biological assays, such as nucleic acid amplification, ligation and sequencing reactions. The novel oligonucleotides comprise a ribonucleic acid domain and a blocking group at or near the 3′ end of the oligonucleotide. These compounds offer an added level of specificity previously unseen. Methods for performing nucleic acid amplification, ligation and sequencing are also provided. Additionally, kits containing the oligonucleotides are also disclosed herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.