Method and device for collection and amplification of circulating nucleic acids

US9644232B2 · kind B2 · utility

Assignee

• General Electric Company · US

Inventors

• Erik Leeming Kvam · Albany, US
• John Richard Nelson · Fryeburg, US
• Gregory Andrew Grossmann · Halfmoon, US
• Ryan Charles Heller · Niskayuna, US
• Erin Jean Finehout · Broomfield, US
• Christopher Michael Puleo · Niskayuna, US
• William Patrick Waters · Scotia, US

Key dates

• Filing date: Jul 26, 2013
• Grant date: May 9, 2017
• Expiry date: Oct 6, 2033

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/0825
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-cellular fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a biological sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane.