Sequencing reactions with lithium for pulse width control

US9650671B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Andrei G. Fedorov · Atlanta, US
• John Lyle · Fremont, US
• Keith Bjornson · Fremont, US
• Jeremiah Hanes · Woodside, US

Key dates

• Filing date: Mar 9, 2015
• Grant date: May 16, 2017
• Expiry date: Jun 22, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having lithium that control the median pulse width for incorporated nucleotides are disclosed. The levels of lithium are used to control pulse width while allowing other sequencing parameters to remain within a desirable range.