Patent · US Active

Bacterial surface display and screening of thioether-bridge-containing peptides

US9651558B2 · kind B2 · utility

0Cited by
1References
20Claims
0Family size

Assignee

Inventor

Key dates

Filing dateJul 6, 2011
Grant dateMay 16, 2017
Priority date—
Expiry dateFeb 6, 2034

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K2319/50
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

The invention relates to bacterial cell surface display of post-translationally modified heterologous proteins. Provided is an isolated nucleic acid construct encoding a proteinaceous substance comprising, from the N-terminus to the C-terminus, at least (a) an N-terminal a lantibiotic leader sequence; (b) an amino acid sequence of interest to be post-translationally modified to a dehydroresidue- or thioether-bridge containing polypeptide; (c) a hydrophilic cell-wall spanning domain; (d) a sortase recognition motif; (e) a hydrophobic membrane spanning domain and (f) a C-terminal charged membrane anchoring domain. Also provided is a Gram-positive host cell expressing the construct, as well as a library of host cells.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.