DNA analyzer

US9656261B2 · kind B2 · utility

Assignees

• LEIDOS INNOVATIONS TECHNOLOGY, INC. · US
• ZYGEM CORPORATION LTD. · NZ
• University of Virginia Patent Foundation · US

Inventors

• H. Randall Bell · Arlington, US
• Joan Bienvenue · Fredericksburg, US
• John W. Pettit · Derwood, US
• James P. Landers · Charlottesville, US
• Jessica V Norris · Palmyra, US
• Orion N. Scott · Charlottesville, US
• Daniel J. Marchiarullo · Morristown, US
• Daniel C. Leslie · Brookline, US

Key dates

• Filing date: Mar 4, 2011
• Grant date: May 23, 2017
• Expiry date: Mar 4, 2031

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/4186
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Aspects of the disclosure provide a microfluidic chip to facilitate DNA analysis. The microfluidic chip includes a first domain configured for polymerase chain reaction (PCR) amplification of DNA fragments, a dilution domain coupled to the first domain to dilute a PCR mixture received from the first domain, and a second domain that is coupled to the dilution domain so as to receive the amplified DNA fragments. The second domain includes a separation channel that is configured to perform electrophoretic separation of the amplified DNA fragments. In addition, the disclosure provides a DNA analyzer to act on the microfluidic chip to perform an integrated single chip DNA analysis.