Scalable process for protein purification
US9657065B2 · kind B2 · utility
1Cited by
3References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 14, 2015 |
| Grant date | May 23, 2017 |
| Priority date | — |
| Expiry date | Jan 14, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/18151
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a process for the purification recombinantly expressed, self-assembled VLP from the homogenate of a bacterial host, wherein the process can be scaled up to a commercial production scale in a cost effective manner. The process comprises a first chromatography using an anion exchange matrix, a second chromatography using hydroxyapatite and, optionally, a size exclusion chromatography. VLP preparations obtained by the process of the invention are essentially free of endotoxin contaminations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.