Reaction buffer for microarray
US9657337B2 · kind B2 · utility
0Cited by
7References
5Claims
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Key dates
| Filing date | Dec 29, 2006 |
| Grant date | May 23, 2017 |
| Priority date | — |
| Expiry date | May 17, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Embodiments of the present invention relate to a buffer composition for an integrated nucleic acid amplification and hybridization reaction. The buffer comprise about 50-200 mM of a salt, about 10-30 mM Tris-HCl, about 2-10M Water soluble magnesium salt, about 0.05-1.5% surfactant, about 0.05-0.15 mg/ml stabilizing protein about 50-300 nM of one or more primers, about 20-150 uM of one or more dNTPs, about 5-15% glycerine, about 0.5-1.5% formamide and at least about 5 unit/ml polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.