Isotopic labeling for the measurement of global protein levels and turnover in vivo

US9658213B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Stanley B. Prusiner · San Francisco, US
• John C. Price · Longmont, US
• Sina Ghaemmaghami · San Francisco, US
• Al Burlingame · Sausalito, US
• Shenheng Guan · Palo Alto, US

Key dates

• Filing date: Jun 17, 2011
• Grant date: May 23, 2017
• Expiry date: Apr 15, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6848
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins.