Signal peptide fusion partners facilitating listerial expression of antigenic sequences and methods of preparation and use thereof

US9663557B2 · kind B2 · utility

Assignee

• Aduro BioTech Inc. · US

Inventors

• Peter Lauer · Albany, US
• William G. Hanson · Walnut Creek, US
• Justin Skoble · Berkeley, US
• Meredith Lai Ling Leong · Oakland, US
• Marcella Fasso · Richmond, US
• Dirk G. Brockstedt · Richmond, US
• Thomas W. Dubensky, Jr. · Piedmont, US

Key dates

• Filing date: Dec 27, 2013
• Grant date: May 30, 2017
• Expiry date: Apr 4, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/40
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides nucleic acids, expression systems, and vaccine strains which provide efficient expression and secretion of antigens of interest into the cytosol of host cells, and elicit effective CD4 and CD8 T cell responses by functionally linking Listerial or other bacterial signal peptides/secretion chaperones as N-terminal fusion partners in translational reading frame with selected recombinant encoded protein antigens. These N-terminal fusion partners are deleted (either by actual deletion, by mutation, or by a combination of these approaches) for any PEST sequences native to the sequence, and/or for certain hydrophobic residues.