Patent · US Active

Chromatography ligand comprising domain C from Staphylococcus aureus protein A for antibody isolation

US9663559B2 · kind B2 · utility

4Cited by

5References

18Claims

0Family size

Assignee

GE Healthcare Bio-Science AB · US

Inventors

Martin Hall · Uppsala, SE
Sture Larsson · Uppsala, SE
Andreas Muranyi · Uppsala, SE
Gustav Rodrigo · Uppsala, SE
Jinyu Zou · Uppsala, SE
Per-Mikael Aberg · Uppsala, SE

Key dates

Filing date	Mar 7, 2016
Grant date	May 30, 2017
Priority date	—
Expiry date	Mar 7, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/55
WIPO fieldChemical engineering
WIPO sectorChemistry

Abstract

The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.