Nucleic acid purification method

US9663779B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Roland Fabis · Leverkusen, DE
• Jan Petzel · Tübingen, DE
• Sabine Scheltinga · Krefeld, DE

Key dates

• Filing date: Dec 23, 2009
• Grant date: May 30, 2017
• Expiry date: Jun 12, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/101
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for purifying nucleic acids from a sample containing nucleic acids, the method comprising at least the following steps: a. bringing the sample containing nucleic acids into contact with a nucleic acid binding phase comprising protonatable groups, wherein the protonatable groups have a pKs value of 9 to 12; b. binding the nucleic acids to the nucleic acid phase at a pH (binding pH) that is at least one pH unit less than the pKs value of at least one of the protonatable groups; c. eluting the nucleic acids at a pH greater than the binding pH but at least one pH unit less than the pKs value of at least one of the protonatable groups. Also disclosed are corresponding kits and nucleic acid binding phases that can be used for purifying nucleic acids.