Patent · US Active

Optimized expression cassette for expressing a polypeptide with high yield

US9663797B2 · kind B2 · utility

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14Claims

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Assignee

Novartis AG · CH

Inventors

Zorica DRAGIC · Basel, CH
Sabine Geisse · Weil am Rhein, DE
Rita Schmitz · Saint-Marcet, FR
Burkhard Wilms · Weil am Rhein, DE

Key dates

Filing date	Nov 18, 2013
Grant date	May 30, 2017
Priority date	—
Expiry date	Nov 18, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2830/30
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is based on the finding that the combination of a specific 5′UTR polynucleotide sequence (see SEQ ID NO 1) and the hCD33 secretory leader sequence in an expression cassette for expressing a polypeptide of interest results in a surprisingly better expression level of the polypeptide of interest compared to prior art expression cassettes. Based on this finding, the present invention inter alia provides novel expression cassettes, expression vectors and methods for producing a polypeptide of interest with high yield.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.