Expression method

US9663798B2 · kind B2 · utility

Assignee

• BASF SE

Inventors

• Lukas Maksym · Cologne, DE
• Ramona Knab · Neuss, DE
• Stefan Evers · Mettmann, DE
• Karl-Heinz Maurer · Erkrath, DE
• Johannes Bongaerts · Dormagen, DE

Key dates

• Filing date: Apr 5, 2012
• Grant date: May 30, 2017
• Expiry date: Mar 26, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.