Patent · US Active

Method for filtering a biological sample

US9663813B2 · kind B2 · utility

0Cited by

1References

21Claims

0Family size

Assignee

BIOCARTIS, NV · BE

Inventors

Roel Penterman · Tisselt, BE
Bart Van Meerbergen · Sint-Job-in-'t-Goor, BE

Key dates

Filing date	Jun 26, 2013
Grant date	May 30, 2017
Priority date	—
Expiry date	Aug 20, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N1/06
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; and (d) washing the filter with a detergent based wash buffer to selectively solubilize proteins originating from the eukaryotic cells retained by the filter, by passing the detergent based wash buffer through the filter, to remove protein clogs from the filter in order to allow an additional step (c) of filtering said lysed sample.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.