Thifensulfuron hydrolase gene tsmE and uses thereof
US9669247B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 17, 2012 |
| Grant date | Jun 6, 2017 |
| Priority date | — |
| Expiry date | Feb 17, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC02F2101/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the field of applied and environmental microorganism and agriculture. Disclosed are a thifensulfuron hydrolase gene tsmE and uses thereof. The thifensulfuron hydrolase gene tsmE has a nucleotide sequence of SEQ ID NO. 1, full length of 1194 bp, and G+C content of 51.09%, and encodes 398 amino acids with an amino acid sequence of SEQ ID NO. 2. The thifensulfuron hydrolase TsmE provided by the present invention can degrade completely 100 mg/L thifensulfuron within 1 hour into the herbicidally inactive product thiophenesulfonic acid; in addition, the TsmE also degrade completely 100 mg/L haloxyfop-R-methyl within 1 hour. Therefore, the thifensulfuron hydrolase gene tsmE is useful in construction of thifensulfuron-resistant transgenic crops and bioremediation of thifensulfuron or haloxyfop-R-methyl-contaminated environments.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.