Modified biotin, mutant streptavidin, and use thereof
US9670255B2 · kind B2 · utility
3Cited by
10References
8Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 18, 2014 |
| Grant date | Jun 6, 2017 |
| Priority date | — |
| Expiry date | Feb 18, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/36
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
It is an object of the present invention to provide a mutant streptavidin with a reduced affinity for natural biotin, and also to provide a modified biotin having a high affinity for the mutant streptavidin with a reduced affinity for natural biotin. According to the present invention, there is provided a reagent kit for use in treatments or diagnoses, which comprises: (a) a mutant streptavidin with a reduced affinity for natural biotin or biocytin; and a modified biotin having a high affinity for the above-described mutant streptavidin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.