Enzyme substrate comprising a functional dye and associated technology and methods
US9670476B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 10, 2013 |
| Grant date | Jun 6, 2017 |
| Priority date | — |
| Expiry date | Oct 10, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07D263/60
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.