Mitochondrial expression vector and method for the transformation of mitochondria
US9670505B2 · kind B2 · utility
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7Claims
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Key dates
| Filing date | Mar 26, 2015 |
| Grant date | Jun 6, 2017 |
| Priority date | — |
| Expiry date | Mar 26, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2820/60
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a mitochondrial expression vector which may comprise a gene to be expressed and/or a selection marker and a mitochondrial region, and a method for inserting a DNA to be expressed into mitochondria of mammalian cells, wherein the method may comprise the steps: (i) construction of a mitochondrial expression vector or a mitochondrial genome, (ii) reversible induction of megamitochondria and (iii) transfection of the megamitochondria by means of a physical transfection method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.