Methods for reducing accumulation of lactate during culturing and method for producing polypeptide

US9670519B2 · kind B2 · utility

Assignee

• Biocon Ltd. · IN

Inventors

• Ruchika Srivastava · Bengaluru, IN
• Sneha Lakshmandas Hemdev · Bengaluru, IN
• Ankur Bhatnagar · Bengaluru, IN
• Saravanan Desan · Bengaluru, IN
• Anuj Goel · Bengaluru, IN
• Harish Iyer · Bengaluru, IN
• Vana Raja · Hosur, IN
• Lavanya Rao · Bengaluru, IN

Key dates

• Filing date: Apr 27, 2012
• Grant date: Jun 6, 2017
• Expiry date: Apr 27, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2500/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.