Enrichment of nucleic acids by complimentary capture

US9670527B2 · kind B2 · utility

Assignee

• THE JOHNS HOPKINS UNIVERSITY · US

Inventors

• Bert Vogelstein · Baltimore, US
• Kenneth W. Kinzler · Baltimore, US
• Nickolas Papadopoulos · Towson, US
• Jian Wu · Baltimore, US

Key dates

• Filing date: Jun 28, 2012
• Grant date: Jun 6, 2017
• Expiry date: Mar 7, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2565/518
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Assays can be used to detect mutations found in neoplasms of the pancreas, as well as for other neoplasms and other uses. Nucleic acids can be captured from body fluids such as cyst fluids. Thousands of oligonucleotides can be synthesized in parallel, amplified and ligated together. The ligated products can be further amplified. The amplified, ligated products are used to capture complementary DNA sequences, which can be analyzed, for example by massively parallel sequencing.