Nucleic acid sequencing by electrochemical detection

US9670538B2 · kind B2 · utility

Assignee

• IBIS BIOSCIENCES, INC. · US

Inventors

• Jeffrey B. Huff · Carlsbad, US
• Graham Davis · Manalapan, US
• Mark A. Hayden · Ingleside, US
• David J. Ecker · Encinitas, US
• Dan Wang · Beijing, CN
• Gordon Bruce Collier · Arnprior, CA

Key dates

• Filing date: Aug 3, 2012
• Grant date: Jun 6, 2017
• Expiry date: May 4, 2034

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided herein is technology relating to sequencing nucleic acids and particularly, but not exclusively, to devices, methods, and systems for sequencing-by-synthesis using changes in pH to monitor base addition. In some embodiments the electrochemical hydrogen ion sensor is a microfabricated mixed metal oxide electrode and in some embodiments the electrochemical hydrogen ion sensor is a membrane electrode. Moreover, in some embodiments the device further comprises a reference electrode. Performing the sequencing reaction involves moving solutions and other fluids (e.g., samples, nucleotide solutions, wash solutions) into and out of the reaction vessel. Thus, in some embodiments, the device further comprises a tube or other transport mechanism or pathway attached to the reaction vessel.