Biochip comprising covalently immobilized bioactive molecules through organic couplers thereon
US9671361B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 2014 |
| Grant date | Jun 6, 2017 |
| Priority date | — |
| Expiry date | Oct 23, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/28
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a biochip comprising an electrode having a titania coating layer on its surface; an organic coupler comprising two or more carboxylic acid groups and capable of transporting electrons; and bioactive molecules, wherein the organic coupler is covalently bonded to a hydroxyl group of titania on the electrode surface through one carboxylic acid group, and to the bioactive molecules through other one or more carboxylic acid groups, a method for analyzing target molecules using the biochip, a method for diagnosing the development of diseases using the biochip, an electrode provided with a titania coating layer on its surface to which an organic coupler, comprising 2 or more carboxylic acid groups and capable of transporting electrons, is bound, wherein the organic coupler is covalently bonded to a hydroxyl group of titania on the electrode surface through one carboxylic acid group, and a method for preparing the electrode provided with a mesoporous titania coating layer, the method comprising coating a mixed solution of a titania precursor and a template polymer on the top of the electrode, and calcinating the coated electrode under an air flow condition.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.