Heparin affinity tag and applications thereof
US9676816B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2014 |
| Grant date | Jun 13, 2017 |
| Priority date | — |
| Expiry date | Jan 21, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/50
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.