Patent · US Active

Detection of methicillin-resistant Staphylococcus aureus

US9677142B2 · kind B2 · utility

0Cited by

40References

17Claims

0Family size

Assignee

ELITECHGROUP B.V. · NL

Inventors

Irina A. Afonina · Mill Creek, US
Yevgeniy S. Belousov · Mill Creek, US
Walt Mahoney · Woodinville, US

Key dates

Filing date	May 24, 2012
Grant date	Jun 13, 2017
Priority date	—
Expiry date	Aug 6, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/158
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present methods pertain to amplifying and/or detecting Staphylococcus aureus (“SA”) and methicillin-resistant Staphylococcus aureus (“MRSA”) nucleic acids based on a combined detection of ldh1 as a SA marker and mecA as a MRSA marker. In certain embodiments the methods also pertain to amplifying and/or detecting one or more SCCmec integration sites or bridge regions. Primers and probes are suitable to be used in the present methods to detect SA and MRSA simultaneously in a single reaction or in separate reactions. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (“FRET”), radiolabels, enzyme labels, and the like.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.