Patent · US Active

Method for activating a nucleic acid for a polymerase reaction

US9683255B2 · kind B2 · utility

0Cited by

323References

14Claims

0Family size

Assignee

QIAGEN GmbH · DE

Inventors

Christian Korfhage · Hilden, DE
Dirk Löffert · Mülheim an der Ruhr, DE

Key dates

Filing date	Sep 11, 2006
Grant date	Jun 20, 2017
Priority date	—
Expiry date	Jul 5, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6844
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention concerns a method for activating a nucleic acid for a polymerase reaction with the steps: (a) Heating a nucleic acid to a temperature of 55° C. to 80° C., (b) cooling the nucleic acid to a temperature at which a polymerase shows no substantial decrease in activity, and (c) starting the polymerase reaction by the addition of a heat-labile polymerase to the nucleic acid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.