Detection of target nucleic acid sequence by PTO cleavage and extension-dependent signaling oligonucleotide cleavage
US9683259B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 16, 2013 |
| Grant date | Jun 20, 2017 |
| Priority date | — |
| Expiry date | Aug 30, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the detection of a target nucleic acid sequence by a PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Cleavage assay (PCE-SC assay). The present invention is carried out in such a manner that the extended strand is produced on the CTO having arbitrary sequences as templates depending on the presence of target nucleic acid sequences and in turn the SO as probes is hybridized with the extended strand to give signal. The present invention employs a series of reactions including PTO hybridization and cleavage, CTO hybridization and extension, and SO hybridization and cleavage, which is responsible for the highly enhanced specificity of the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.