Apparatus and methods for multiphoton microscopy
US9687152B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 5, 2012 |
| Grant date | Jun 27, 2017 |
| Priority date | — |
| Expiry date | Mar 14, 2034 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61N5/0616
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
A multiphoton microscope is provided. The microscope includes: an excitation source for providing an optical excitation beam at an excitation wavelength λ; a scanner for scanning the excitation beam on a sample; an objective for irradiating the sample with the excitation beam scanned by the scanner and for collecting an emission beam from the sample; a first detector for detecting a plurality of multiphoton signals; and an emission light path allowing transmission from the objective to the first detector a wavelength band limited to greater than or equal to λ/2 and less than λ, wherein the plurality of multiphoton signals have wavelengths within the wavelength band; wherein the plurality of multiphoton signals com-prises a first multiphoton signal and a second multiphoton signal of different types. Fast image capture rate multiphoton microscopes for in vivo imaging, as well as photothermolysis methods using the microscopes are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.