Ultrafiltration for preparing outer membrane vesicles
US9687789B2 · kind B2 · utility
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20References
15Claims
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Assignee
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Key dates
| Filing date | Jul 15, 2004 |
| Grant date | Jun 27, 2017 |
| Priority date | — |
| Expiry date | Jan 8, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/36
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
In place of a step of centrifugation during preparation of outer membrane vesicles (OMVs) from bacteria, the invention utilizes ultrafiltration. This allows much larger amounts of OMV-containing supernatant to be processed in a much shorter time. Thus the invention provides a process for preparing bacterial OMVs, comprising a step of ultrafiltration. The ultrafiltration step is performed on an aqueous suspension of OMVs after they have been prepared from bacteria and the OMVs remain in suspension after the filtration step. The invention is particularly useful for preparing OMVs from Neisseria meningitidis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.