Plant-based recombinant butyrylcholinesterase production methods

US9688970B2 · kind B2 · utility

Assignee

• Kentucky BioProcessing, Inc. · US

Inventors

• Greg Pogue · Austin, US
• Ernie Hiatt · Maceo, US
• Romy Kandzia · Bodenwerder, DE
• Stefan Werner · Halle (Saale), DE
• Frank Thieme · Halle (Saale), DE
• Tsafrir Mor · Tempe, US
• Steven D. Hume · Owensboro, US

Key dates

• Filing date: Jul 31, 2014
• Grant date: Jun 27, 2017
• Expiry date: May 31, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y301/01008
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.