Methods of introducing nucleic acids into cellular DNA

US9688994B2 · kind B2 · utility

Assignee

• President and Fellows of Harvard College · US

Inventors

• Marc Joseph LAJOIE · Seattle, US
• Christopher Gregg · Cambridge, US
• Joshua A. Mosberg · Cambridge, US
• George M. Church · Brookline, US

Key dates

• Filing date: Jul 30, 2013
• Grant date: Jun 27, 2017
• Expiry date: Jul 30, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of introducing a nucleic acid sequence into a cell is provided where the cell has impaired or inhibited or disrupted DnaG primase activity or impaired or inhibited or disrupted DnaB helicase activity, or larger or increased gaps or distance between Okazaki fragments or lowered or reduced frequency of Okazaki fragment initiation, or the cell has increased single stranded DNA (ssDNA) on the lagging strand of the replication fork including transforming the cell through recombination with a nucleic acid oligomer.