Method of dual-adapter recombination for efficient concatenation of multiple DNA fragments in shuffled or specified arrangements
US9689012B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 12, 2011 |
| Grant date | Jun 27, 2017 |
| Priority date | — |
| Expiry date | Jan 29, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/66
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods of assembling a plurality of genetic units to form synthetic genetic constructs. This method involves appending universal adapter oligonucleotides and flexible adapter oligonucleotides to the 5′ and 3′ ends of separate genetic units to be assembled to form separate dual extended genetic units. The dual extended genetic units are assembled together via homologous recombination between the flexible adapter oligonucleotide portions of the dual extended units to form synthetic genetic constructs. The present invention further relates to synthetic genetic constructs formed using the methods of the present invention, and vectors, cells, and organisms containing such synthetic genetic constructs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.