Method for measuring beta-lactam antibiotics

US9689021B2 · kind B2 · utility

Assignees

• UNIVERSITÉ DE LIÈGE · BE
• UNIVERSITÉ CATHOLIQUE DE LOUVAIN · BE
• UNIVERSITÉ LIBRE DE BRUXELLES · BE
• UNIVERSITÉ DE MONS-HAINAUT · BE

Inventors

• Alain Brans · Beaufays, BE
• Bernard Joris · Spa, BE
• Michael Delmarcelle · Walhain, BE
• Jacqueline Marchand · Marcinelle, BE
• Paul Tulkens · Nivelles, BE
• Catherine Hammaecher · Pinon, FR
• Erik Goormaghtigh · Enghien, BE
• Joel De Coninck · Mons, BE

Key dates

• Filing date: Oct 15, 2012
• Grant date: Jun 27, 2017
• Expiry date: Oct 15, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/986
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to methods for determining the concentration of a free beta-lactam antibiotic in a biological sample. In particular, the present invention relates to methods for measuring a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at least one class C beta-lactamase, a functional fragment or variant thereof; (b) providing at least one biological sample; (c) contacting said at least one class C beta-lactamase with said at least one biological sample; and (d) determining the concentration of said free beta-lactam antibiotic in said at least one biological sample.