Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

US9689047B2 · kind B2 · utility

Assignee

• QIAGEN GAITHERSBURG INC. · US

Inventors

• Dominic O'Neil · Hilden, DE
• Irina Nazarenko · Gaithersburg, US
• Holly Basham · Reisterstown, US
• Arvind Virmani · Germantown, US
• Shiuli Agarwal · Somerville, US
• Mridula Shukla · Jersey City, US

Key dates

• Filing date: Jan 28, 2011
• Grant date: Jun 27, 2017
• Expiry date: Jan 28, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6806
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step using two separate sequence-specific polynucleotide probes. Also provided are nucleic acids comprising SEQ ID NO: 1 to SEQ ID NO: 727 and nucleic acid probes and probe sets comprising the same.