Method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme

US9695461B2 · kind B2 · utility

Assignee

• Henkel AG & Co. KGaA · DE

Inventors

• Petra Siegert · Haan, DE
• Stefan Evers · Mettmann, DE
• Marion Merkel · Cologne, DE
• Nina Mussmann · Willich, DE
• Hendrik Hellmuth · Darmstadt, DE
• Timothy O'Connell · Fürstenfeldbruck, DE
• Karl-Heinz Maurer · Erkrath, DE
• Ulrich Schwaneberg · Waiblingen, DE
• Felix Jakob · Lüdenscheid, DE
• Ronny Martinez · Aachen, DE
• Brian Laufs · Frechen, DE
• Ayhan Aydemir · Goldkronach, DE
• Thomas Weber · Dormagen, DE

Key dates

• Filing date: Mar 6, 2014
• Grant date: Jul 4, 2017
• Expiry date: Mar 6, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/96
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The stabilization of a hydrolytic enzyme in a liquid preparation is to be improved through a component that stabilizes the hydrolytic enzyme. This is accomplished by a method for adapting a hydrolytic enzyme to a component that stabilizes the hydrolytic enzyme, comprising the following method steps: a) providing a hydrolytic enzyme (starting enzyme) and a component that stabilizes the hydrolytic enzyme and that comprises a reversible inhibitor of the hydrolytic enzyme; b) changing the amino acid sequence of the hydrolytic enzyme in at least one position, by substitution, deletion or insertion; c) determining the relative activity of the hydrolytic enzyme from step b) and the relative activity of the starting enzyme in a liquid preparation; d) selecting the hydrolytic enzyme that has a reduced relative activity by comparison with the relative activity of the starting enzyme.