Method and device for uniformly treating adherent cells

US9701954B2 · kind B2 · utility

Assignee

• Lonza Cologne GmbH · DE

Inventors

• Thomas Koblizek · Oettingen, DE
• Andreas Heinze
• Timo Gleissner · Euskirchen, DE
• Herbert Mueller-Hartmann · Cologne, DE
• Andreas Wirth · Simbach, DE

Key dates

• Filing date: Jun 21, 2011
• Grant date: Jul 11, 2017
• Expiry date: Jul 12, 2032

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61N1/327
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied. The invention further relates to a device 58 comprising at least three electrodes 63, 64, which are connected to at least one voltage source by means of at least one switching device 59, 60, 61, 62, wherein at least five electrodes 63, 64 are connected to the at least one voltage source by means of four switching devices 59, 60, 61, 62, wherein at least two electrodes 63, 64 are connected to the voltage source by means of a common switching device 59, 60, 61, 62. In conjunction with the method described above, the device according to the invention allows the efficient and uniform treatment of adherent cells usin…