In vitro production of DNA minicircles comprising less than 250 base pairs
US9701956B2 · kind B2 · utility
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Key dates
| Filing date | Nov 20, 2013 |
| Grant date | Jul 11, 2017 |
| Priority date | — |
| Expiry date | Nov 20, 2033 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K48/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for the in vitro production of DNA minicircles includes steps of: a) providing nicked double-stranded oligodeoxynucleotides bunt-ended substrates having at least one phosphorylated 5′ end, b) performing a ligase-mediated circularization on a reaction mixture including the nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.