Nucleosome-excluding sequences (NES) as a DNA spacer in vectors results in prolonged transgene expression in eukaryotic cells

US9701981B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Jiamiao Lu · Mountain View, US
• Mark A. Kay · Los Altos, US
• Andrew Fire · Stanford, US
• Lia E. Gracey Maniar · Menlo Park, US

Key dates

• Filing date: Mar 11, 2014
• Grant date: Jul 11, 2017
• Expiry date: Mar 11, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/85
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The silencing effect of a spacer sequence, for example a bacterial backbone sequence in a plasmid or other episomal vector, on transgene expression is reversed by engineering of the spacer to include nucleosome exclusion sequences.